A scan has revealed that Toby Roland-Jones has sustained a stress fracture in his lower back.
The injury to the 29-year-old's L5 vertebra means he will take no further part in the domestic season for his county Middlesex.
Roland-Jones impressed on his breakthrough into the England Test team this summer, taking 17 wickets in four matches against South Africa and West Indies.
The England selectors will announce the squad for the Ashes tour to Australia at the Oval next Wednesday.